摘要
基于分子标记的茄子(Solanum melongena)杂交种子纯度快速检测技术体系的建立对茄子杂交品种的研究具有重要的意义。本研究以茄子杂交品种"苏崎3号"、"苏崎4号"及其双亲为试验材料,采用NaOH裂解法快速提取DNA与SSR标记分析相结合的方法,对茄子杂交品种种子纯度的鉴定技术进行了研究。结果表明,利用NaOH裂解法提取的DNA能够进行正常的SSR分析。从46对SSR引物中各筛选出1个共显性标记,对"苏崎3号"和"苏崎4号"种子进行群体检测,发现分子标记鉴定结果与田间鉴定结果一致。研究结果表明NaOH裂解法和SSR标记分析相结合可实现对茄子杂交品种种子纯度的快速和准确鉴定。
It is important to construct a molecular marker based detection system of seed purity for eggplant hybrid. In this paper, two eggplant hybrids, ＇Suqi 3＇ and ＇Suqi 4＇, and their parents were used as plant materials. Rapid DNA extraction by NaOH method and SSR analysis were used to construct a system for the identification of seed purity of eggplant hybrids. The data showed that the DNA quality was suitable for SSR amplification. By screening 46 pairs of SSR primers, two co-dominance SSR markers were identified for ＇ Suqi 3＇ and ＇ Suqi 4＇ respectively. Further seed purity identification of two hybrid populations by SSR markers showed the coincident results of field identification. Above results indicated that the combination of DNA extraction by NaOH method and SSR analysis is a rapid and accurate method for the detection of eggplant hybrid seed purity.
出处
《分子植物育种》
CAS
CSCD
北大核心
2013年第6期790-794,共5页
Molecular Plant Breeding
基金
江苏省农业科技自主创新资金(CX(13)2003)
江苏省自然科学基金(BK2011675)共同资助
