红掌ANR基因克隆及其表达与佛焰苞颜色的相关性分析

Cloning of ANR Gene from Anthurium and Relativity Analysis of Its Expression with the Spath Color

花青素是植物颜色形成的重要成分之一。不同红掌品种中的花青素含量与佛焰苞颜色密切相关。花青素还原酶（㈣是植物花青素调控代谢途径中催化产生原花青素的一个重要基因。为了探明ANR对花青素合成的作用，本研究根据从抑制差减文库中获得的基因片段，采用RACE技术克隆红掌ANR基因cDNA全长序列。BLAST结果显示，红掌ANR基因全长核苷酸序列与己报道的植物（葡萄，茶树，毛果杨等）同源性为72％~75％，氨基酸序列的同源性则达到78％一79％。RT—PCR结果表明ANR在不同颜色的红掌佛焰苞中表达有差异，其表达量与花青素含量成负相关。

Anthocyanidin is an important content for plant color appearence. Caltivars ofA nthurium andraeanum display varies spathe colors have different concentrations of anthocyanidin. In anthocyanin biosynthetic pathway, Anthocyanidin Reductase （ANR） is a key enzyme to catalyze anthocyanidins to proanthocyanidin （PC）. In order to verify the regulation of ANR in anthocyanin synthesis, we cloned the whole eDNA sequence of ANR by RACE technology according to the known ANR fragment from SSH library. BLAST results showed that A nA NR had high similarity, 72%-75%, with reported ANR gene from Populus trichocarpa, Camellia sinensis and Vitis vinifera. RT-PCR results showed that in anthurium cultivars with different spathe colors, the expression level of A nA NR was negatively related with the accumulation of anthocyanidin.