肝硬化大鼠肾小球收缩敏感度增高参与肝肾综合征肾小球滤过率下降的机制

Hyper-responsiveness of Glomeruli to Vasoconstrictor in Cirrhotic Rats Participates in the Decreased Glomerular Filtration Rate in Hepatorenal Syndrome

目的通过体外测量肝硬化大鼠肾小球对缩血管物质的敏感度,探讨肝硬化时易于发生肝肾综合征的机制。方法雄性SD大鼠被随机分为生理盐水对照组、肝硬化组(四氯化碳法)。12周结束时颈椎脱臼法处死大鼠,筛网法分离肾小球并进行活性鉴定。体外应用FITC标记的菊粉,荧光分光光度计下进行肾小球菊粉容积测量(GIS),并于共聚焦显微镜下测量肾小球周长,观察两组肾小球对内皮素-1缩血管作用敏感度的差异。结果内皮素-1刺激后,肝硬化组肾小球的GIS变化百分比及肾小球周长变化百分比与对照组相比显著增加(17.26%±4.80%,10.50%±2.83%,P=0.001;11.19%±4.14%,8.53%±3.05%,P=0.007)。结论肝硬化大鼠肾小球对缩血管物质的敏感度是增高的,这可能是肝硬化晚期易于发生肝肾综合征的机制之一。

Objective To explore the mechanism of cirrhosis prone to hepatorena] syndrome by measuring the reactivity of glomeruli to endothelin - 1 （ ET - 1 ） in vitro. Methods Male sprague dawley （SD） rats were randomly divided into saline control group, cirrhosis group（ carbon tetrachloride, CC14 ）. At the end of 12 weeks, rats were put to death by cervical dislocation method. Glomeruli was isolated by the gradual sieving technique, whose activity was identified. By measuring glomerular inulin space （GIS） with FITC -labeled inulin, and glomerular circumference with eonfoeal microscopy, we observed the different reactivity of glomeruli to ET- 1 between two groups. Results Compared to control group, the change percentage of GIS and of glomerular circumference in cirrhotic group to endothelin stimu- lation was significantly larger （17.26% ：i：4.80%,10.50% 2.83%,P=0.001;11. 19% ＋4. 14%,8.53% ＋3.05% ,P =0.007）. Conclusion The reactivity of glomerular to ET - 1 in cirrhotic rats is increased, which may be one of the mechanisms of cirrhosis prone to hepatorenal syndrome.