白藜芦醇对腹膜透析液作用下人腹膜间皮细胞线粒体活性氧产生的影响

Effect of Resveratrol of Reactive Oxygen Species in Human Peritoneal Mesothelial Cells Exposed to Glucose-based Peritoneal Dialysis

目的白藜芦醇对腹膜透析液作用下人腹膜间皮细胞线粒体活性氧产生的影响。方法体外培养人腹膜间皮细胞株第5～10代（HMrSV5，DMEM／F12培养基含10％胎牛血清）用于实验研究。MTT检测细胞活力。实验分组：阴性对照组（培养基）、阳性对照组（加入Rotenone，细胞线粒体呼吸链酶复合体I抑制剂）、实验组（1．5％葡萄糖腹膜透析液）、丁预组（白藜芦醇＋1．5％葡萄糖腹膜透析液）。流式细胞仪检测总线粒体、呼吸性线粒体、产活性氧线粒体。结果与阴性对照组比较，实验组总线粒体、产活性氧线粒体增加，差异具有统计学意义。与实验组比较，干预组总线粒体、产活性氧线粒体减少，差异具有统计学意义。结论白藜芦醇可降低含葡萄糖腹膜透析液作用人腹膜间皮细胞后活性氧的产生。

Objective To explore the effect of resveratrol of reactive oxygen species in human peritoneal mesothelial cells exposed to glucose- based peritoneal dialysis. Methods Cells （SV40 immortalized human peritoneal mesothelial cell line） were grown in type ] collagen - coated dishes in DMEM/F12 containing 10% FCS. All experiments on immortalized mesothelial cells were performed from pas- sages 5 to 10. Cell viability was assessed using MTT. Cells were divided into 4 groups： negative control （medium） , positive control （ Ro- tenone） , 1.5% glucose -based peritoneal dialysis, resveratrol plus 1.5% glucose- based peritoneal dialysis. Flow cytometric was used to analyze the Mitotracker deep red,Mitotraeker green and MitoSOX. Results Compared to the negative control, Mitotracker green and MitoSOX were significantly increased in the cells exposed to 1.5% glucose -based peritoneal dialysis. In addition, Mitotracker green and MitoSOX were significantly decreased in the cells exposed to resveratrol plus 1.5% glucose - based peritoneal dialysis compared to the cells exposed to 1.5% glucose - based peritoneal dialysis only. Conclusioll Resveratrol can reduce the reactive oxygen species in human peritoneal mesothelial cells exposed to glucose -based peritoneal dialysis.