DAPT对大鼠自体移植静脉桥狭窄的抑制作用

DAPT Inhibits Autogeneic Vein Graft Model in Rats

目的观察DAPT对大鼠自体移植静脉桥狭窄的抑制作用。方法成年Wistar大鼠随机分为实验组、对照组和安慰剂组,每组12只。所有大鼠分离并切取0.5cm长颈外静脉,移植到自体同侧颈总动脉中。分别于术后第1天、第4天和第7天取各组动物各4只,完整取下移植静脉桥,常规石蜡包埋,切片行HE及PCNA和TUNEL免疫组化染色,计算静脉桥中膜增生的厚度和平滑肌细胞PCNA和TUNEL阳性指数,并用Western blot检测SM22和SM MHC变化。结果移植后第7天实验组静脉桥中膜厚度和PCNA阳性指数比对照组和安慰剂组明显较小(P<0.05),但实验组凋亡程度明显高于安慰剂组。Western blot显示静脉桥移植后SM22和SM MHC表达明显降低,syndecan-1表达降低,但DAPT能够促使其表达回复。结论 DAPT能够缓解静脉桥狭窄,并对移植静脉内平滑肌细胞增生有抑制作用。

Objective To discuss if DAPT inhibits autogeneic vein graft model. Methods Thirty - six adult rats used in this study were divided into three groups （ n = 12 for each graup）. The autogenous jugular vein of all rats was grafted into the carotid artery by microsurgery. After the operation, rats in each group were fed in the same way. The control group do nothing. In experiment and placebo group, DAPT and DSMO were administrated to rats by a daily subcutaneous dose of 10mg per kg body weight respectively from day 0 to day 7 post operation. In placebo, at ldays, 4days and 7days after grafting,the grafted from four of each groups vessel were taken out. Results Inti- mal hyperplasia （IH） and anti -proliferating cell nuclear antibody （PCNA） index of smooth muscle cell （SMC）of vein graft in experiment group were lower than those in control and placebo group. And the TUNEL index of smooth muscle cell （SMC） and SM22 and SM MHC in IH of experiment group were higher than those of control group. Conclusion DAPT can ameliorate the stricture of the vein graft model.