Shh和Gli1在宫颈癌中的表达与临床意义

Expression of Shh and Gli1 in Cervical Cancer and Its Clinical Significance

目的探讨hedgehog信号通路Shh蛋白及其下游转录因子Gli1蛋白在宫颈癌组织中的表达及其意义。方法采用免疫组化S-P法分别检测83例子宫颈鳞状细胞癌(SCC)、39例子宫颈上皮内瘤样病变(CIN)和23例正常宫颈组织(NC)中Shh和Gli1蛋白的表达。结果 Shh和Gli1蛋白在正常宫颈组织、CIN及宫颈癌组织的表达呈逐渐上升趋势,3组比较有统计学差异(P<0.01)。Shh和Gli1蛋白表达率与病理分化程度和临床分期有密切关系,且有淋巴结转移的宫颈癌组织的表达率显著高于无淋巴结转移组(P均<0.01)。Shh和Gli1蛋白在宫颈癌中的表达呈正相关(P<0.01)。结论在宫颈癌中Shh蛋白高度表达上调下游转录因子Gli1蛋白,Hh信号通路的激活可能在宫颈癌的发生过程中其重要作用。

Objective To study the expression of Shh protein and downstream transcription factor Glil protein in hedgehog signal transduction pathway in cervical cancer. Methods The expression of Shh and Glil proteins were detected by Immunohistochemieal S - P method in 83 cases of cervical squamous cell carcinoma, 39 cases of cervical intraepithelial neoplasia（CIN） and 23 cases of normal cervi- cal specimens. Results The expression rates of Shh and Glil proteins were raised gradually from normal specimens, CIN to cervical cancer with significantly differences of three group （P 〈 0.01 ）. The expression of Shh and Glil proteins was associated with pathological grades and clinical stage of cervical cancers （ P 〈 0.01 ）. The Shh and Glil positive expression rates in lymph node metastasis were signif- icantly higher than those without lymph node metastasis （ P 〈 0.01 ）. The expression of Shh and Glil had positive correlation （ P 〈 0.01 ）. Conclusion The over-expression of Shh upregulates the expression of the downstream transcription factor Glil protein in cervical cancer. The activation of hedgehog signal transduction pathway may play important role in the development of cervical cancers.