脑干诱发电位对重症急性胰腺炎大鼠脑损伤的诊断作用

The Diagnostic Effect of Brainstem Evoked Potential for Pancreatic Encephalopathy in Rats with Severe Acute Pancreatitis

目的探讨脑干诱发电位对重症急性胰腺炎(severe acute pancreatiti,SAP)大鼠脑损伤的诊断作用.方法 60只成年雄性SD大鼠随机分为假手术(sham operation,SO)组和重症急性胰腺炎(SAP)组.两组均于制模后3、6、12 h测量脑干诱发电位变化;透射电镜观察脑组织超微结构的变化;免疫组化和Western Blot检测脑组织Bax、bcl-2和caspase-3蛋白表达.结果与SO组比较,SAP组各时间点脑干诱发电位均呈下降趋势(P<0.05);透射电镜结果显示SAP组出现明显脑组织损伤变化;SO组Bax、caspase-3蛋白表达显著低于SAP组(P<0.05),bcl-2蛋白表达明显高于SAP组.结论脑干诱发电位对大鼠脑损伤早期诊断灵敏,其结果与大鼠脑损伤程度表现出一致性,可能成为胰性脑病的重要临床诊断指标.

Objective The aim of this study is to demonstrate the diagnostic effect of hrainstem evoked potential for pancreatic encephalopathy in rats with severe acute pancreatitis. Methods Sixty male Sprague-Dawley （SD） rats were randomly divided into two equal groups： a sham-operated （SO） group and a severe acute pancreatitis （SAP） group. Each group was evaluated at 3, 6, and 12 h during the experiment. To detect the brain stem evoked potential change at different time points. The uhrastructure of brain tissue was observed by transmission electron microscope （TEM）. The expressions of the apoptosis-related proteins Bcl-2, Bax and caspase-3 were observed using immunohistochemical and Western Blot technique. Results In SAP group, congestion, edema, inflammatory cell infiltration, mitochondrial swelling and cell apoptosis were apparent. Compared with SO group, the brain stem evoked potential in severe acute pancreatitis group was obviously reduced in SAP group. Compared with SAP group, the expressions of Bcl-2 have increased, whereas the expressions of Bax and caspase-3 have decreased in SO group significantly （P 〈 0.05） . Conclusion Brain stem evoked potential is a sensitive method in detection of rat brain damage. The results showed that the consistency and the damage degree of rats may be important clinical diagnostic index of pancreatic encephalopathy.