高浓度葡萄糖对人树突状细胞CD83、CD86、CD36、CCR7和μ-阿片受体的影响

Effect of high glucose on the expressions of CD83,CD86, CD36,CCR7 and μ-opioid receptor in dendritic cells

目的：观察高浓度葡萄糖对人树突状细胞（DC）受体CD83、CD86、CD36、CCR7和μ-阿片受体（MOR）表达的影响，探讨高血糖在动脉粥样硬化免疫炎症反应中的作用。方法：从人外周血中提取和分离单个核细胞，在含重组人粒细胞-巨噬细胞集落刺激因子和重组人白介素4的RPMI1640完全培养基中培养。7d后未成熟DC分3组，分别在含有D-葡萄糖5mmol／L（NG组）、10mmol／L（TG组）和25mmool／L（HG组）的RPMI1640完全培养基中继续培养48h后，采用流式细胞术检测DC表面CD83、CD86、CD36、MOR和CCR7的变化。结果：TG组和HG组DC表面分子CD36、CCR7、CD83和CD86的阳性率均高于NG组（P〈0．05-P〈0．01），HG组DC表面分子MOR的阳性率高于NG组（P〈0．05），而HG组CD36、CD83和CD86的阳性率亦均明显高于TG组（P〈0．01）。结论：高浓度葡萄糖能促进DC表面分子CD36、CD86、CD83和CCR7的表达。

Objective： The aim of the present study was to investigate the expressions of scavenger receptor CD36, CCR7, CD83, CD86 and μ-opioid receptor （ MOR ） in dendritic cells （ DC ） induced by high glucose, then explore the effect of hyperglycemia on atherosclerosis. Methods：Immature dendritic cells （imDC） were extracted from normal human peripheral blood mono-nuclear cells （PBMC） which were isolated and cuhured with cytokines including GM-CSF and IL-4. After 7 days, the imDC were separated into 3 group,and differently cultured in RPMI1640 medium with either 5 mmol/L D-glucose（ NG group）, 10 mmol/L glucose（ TG group） and 25 mmol/L D-glucose（ HG group） for 48 hours, and the expressions of the surface markers（ CD83, CD86, CD36 and CCR7） in DC were analyzed by flow cytometry. Results： Compared with NG group, the expressions of CD36, CD83, CD86 and CCR7 were significantly upregulated in TG and HG groups （ P 〈 0.05 to P 〈 0.01 ）. Compared with NG group, the expression of MOR was signifieuntly upregulated in HG group （ P 〈 0.05 ）. And compared with TG group, the expressions of CD36, CD83 and CD86 were significantly increased in HG group （ P 〈 0.01 ）. Conclusions ： High glucose can promote the expressions of surface molecular CD36, MOR and CCR7 in DC.