氯化钴对小鼠腹腔巨噬细胞的抑制作用

The inhibition of CoCl2on mouse peritoneal macrophages

目的观察氯化钴(CoCl2)对巨噬细胞体外培养的抑制作用。方法采用小鼠巨噬细胞体外培养法在不同浓度(0.5、5.0、50.0 mg/ml)的CoCl2作用下,应用细胞增殖抑制试验(MTT法)测定体外巨噬细胞的抑制率、应用吖啶橙染色法测定细胞凋亡情况和应用考马斯亮蓝染色法测定总蛋白含量的变化。结果 50.0 mg/ml的CoCl2作用24 h,小鼠腹腔巨噬细胞中蛋白质相对含量为75.2%,与对照组的100%相比,差异有统计学意义(P<0.01);5.0和50.0 mg/ml CoCl2作用48和72 h,巨噬细胞中蛋白质含量与对照组相比,差异均有统计学意义(P<0.01)。采用吖啶橙染色法测定细胞凋亡发现在CoCl2的作用下有明显凋亡现象。结论 CoCl2对小鼠体外巨噬细胞增殖有明显的抑制作用,且有促细胞凋亡和抑制蛋白生成作用。

[ Objective ] To observe the inhibition effect of CoC12 on macrophages cultured in vitro [ Mothods] Adopt the method of culturing the mouse macrophages in vitro, which is under the different concentration level （0.5,5.0,50.0 mg/ml） of CoC12. The inhibition ratio of macrophage in vitro was tested by MTI＂ method,the condition of apoptosis was tested by acridine orange staining method and the change of the total protein content was tested by Commassie blue staining method. [ Result] After exposed the CoCl2 of 50.0 mg/ml 24 h, the relative contents of protein in mouse peritoneal macrophages was 75.2%. Compared to the 100% of the control group , the difference was statistically significant（ P 〈 0.01 ）. After exposed the CoCl2 of 5.0 and 50.0 mg/m148 and 72 h, compared to the control group, the difference of the protein content in macrophages were all statistically significant（ P 〈0.01 ）. The obvious phenomenon of apoptosis was found by acridine orange staining method under the effect of CoCl2. [ Conclusion] The CoCI2 can significantly inhibit the proliferation of the mouse macrophage, promote apoptosis and inhibit the protein generation.