山楂叶悬钩子水提取物对转化生长因子-β刺激的肝星状细胞活化的抑制作用及其机制

Inhibiting effects and mechanism of aqueous extract of Rubus crataegifolius for activation of tHSC/Cl-6 stimulated by TGF-β

[目的]探讨山楂叶悬钩子水提取物（RC—H）对转化生长因子（TGF—β）刺激的肝星状细胞（HSC）活化的影响及其机制．[方法]采用MTT法测定RC-H对大鼠肝星状细胞（tHSC／C1—6）存活率的影响；采用TGF-β促活化tHSC／C1—6细胞，蛋白印迹分析RC-H对TGF-β刺激HSC信号转导的影响．[结果]0～160mg／LRC-H对tHSC／Cl-6存活率无显著影响（P〉0．05），320，640，1280mg／LRC-H显著抑制tHSC／C1—6的存活率（P〈0．05）．40，80，160mg／LRC—H可抑制TGF-β活化tHSC／Cl-6细胞中α-SMA蛋白表达，下调TIMP-1蛋白表达，上调MMP-13蛋白表达，降低p-Erk和p-JNK蛋白表达，对p-p38无显著影响．[结论]RC—H可通过介导MAPK信号通路中Erk和JNK磷酸化而抑制TGF-β刺激的肝星状细胞活化．

OBJECTIVE To study the effects and its mechanism of aqueous extract of Rubus crataegifoli- us （RC-H） for activation of hepatic stellate cells tHSC/C1-6 stimulated by transforming growth factor-β（TGF-β）. METHODS Effects of RC-H on cell viability of tHSC/C1-6 were detected by MTT, and on the signal transduction of tHSC/C1-6 activated by TGF-β were detected by Western blot. RESULTS 0 160 mg/L of RC-H had no obvious effects for the cell viability of tHSC/C1-6 （P〉0.05）, and 320, 640 and 1 280 mg/L of RC-H had significant effects for the cell viability of tHSC/C1-6 （P〈0.05）. 40, 80 and 160 mg/L of RC-H in tHSC/C1-6 activated by TGF-β inhibited the expression of α-SMA and TIMP-1, increased the expression of MMP-13 and decreased the expression of p-Erk and p-JNK, and had no effect on p-p38. CONCLUSION Aqueous extract of RC-H might inhibit the activation of tHSC/C1-6 stimulated by TGF-β via phosphorylation of JNK and Erk in MAPK signal pathway.