辽宁省食源性沙门菌血清分布及耐药分析

Analysis of serotype distribution and drug resistance of foodborne salmonella in Liaoning province

目的监测了解辽宁省食品中沙门菌血清分布和耐药状况,为有效控制沙门菌引起的食源性疾病暴发流行、控制耐药菌株的传播提供参考数据。方法应用GB 4789.4-2010《食品安全国家标准食品微生物学检验沙门菌检验》,对沙门菌分离株进行血清学鉴定,使用微量肉汤稀释法进行耐药测定。结果 35株沙门菌分离株血清分型34株,同为肠道沙门菌,以B群血清型最多,其次为D群。主要血清型为布利丹沙门菌、肠炎沙门菌、圣保罗沙门菌。43株沙门菌药敏试验显示对萘啶酸的耐药率最高(74.4%),其次是四环素、卡那霉素和氨苄青霉素(均为37.2%)。结论辽宁省食源性沙门菌血清型分布呈多样性,所选的16种抗生素药敏试验,部分菌株耐受多种抗生素。建议有关部门对抗生素耐药趋势引起足够重视,加强对饲用抗生素的管理,建立食源性耐药菌的风险评估机制。

Objective To monitor and understand the drug resistance and serological distribution of salmonella in food of Liaoning province, so as to provide reference data to prevent food - borne illness epidemic by Salmonella and control drug resistant strains. Methods According to GB 4789.4 - 2010, the salmonella isolates were conducted serological identification. Micro - broth dilution method was used to determine the drug resistance of these isolates. Results Thirty - four salmonella isolates were identified as the same serotype, salmonella enterica, and most belonged to B group, followed by D group. The main serotypes were S. sendai, S. enteritidis, S. saint -paul. Forty - three salmonella isolates showed highest resistance to nalidixic acid （74.4%） , followed by ampicillin, kanamycin and ampicillin（ all were 37.2% ）. Conclusion In Liaoning province, food - borne salmonella performed diverse serotypes. Part of the salmonella isolates had tolerance to multiple antibiotics. So the relevant departments should strengthen monitoring on drug resistance trend of salmonella and establish risk assessment mechanism of food- borne resistant strains.