北京市食源性沙门菌血清型和分子特征研究

Serotyping and molecular characteristics of Salmonella isolates from food in Beijing

目的分析近年来北京市食源性沙门菌的血清型和脉冲场凝胶电泳(PFGE)图谱特征。方法对2004年-2010年北京市自食品中分离到的100株沙门菌进行血清分型和PFGE分型。结果 100株沙门菌共分为25个血清型,其中位居前三位的血清型是肠炎沙门菌、德尔卑沙门菌和阿贡纳沙门菌,分别占18%,17%,10%。在分离来源上90%的沙门菌分离自肉类制品。100株菌共分为62种PFGE带型,带型分布较为分散,优势带型不明显。其中肠炎沙门菌共有9种PFGE带型,其优势带型为JEGX01.CN0003;德尔卑沙门菌共有11种PFGE带型,其优势带型为JDPX01.CN0001和JDPX01.CN0032。PFGE图谱的聚集性与沙门菌的血清型有一定的联系,相同血清型沙门菌的PFGE带型聚集成簇。结论北京市食源性沙门菌的血清型和PFGE带型分布广泛,生肉制品是其主要的来源。

Objective To study the serotypes and molecular characteristics of Salmonella isolated from food by pulsed - field gel electrophoresis （PFGE） in Beijing in recent years. Methods A total of 100 Salmonella strains isolated from food from 2004 to 2010 were identified by biochemical tests and serotyping. The isolates were further typed by PFGE. Results The 100 Salmo- nella isolates belonged to 25 serotypes, including S. Enteritidis（ 18% ）, S. Derby（ 17% ） and S. Agona（ 10% ） as the dominant serotypes. 90% of the Salmonella strains were isolated from meat products. Totally, 62 PFGE patterns were identified with dif- fused distribution. No dominant PFGE patterns were obviously identified. Nine PFGE patterns were observed in S. Enteritidis i- solates with JEGX01. CN0003 as the dominant.pattern. Eleven PFGE patterns were observed in S. Derby isolates with JDPX01. CN0001 and JDPX01. CN0032 as the dominant patterns. There was some correlation between PFGE patterns and serotypes of Salmonella. The PFGE patterns of the same Salmonella serotype were clustered together. Conclusion The serotypes and PFGE patterns of Salmonella isolated from food in Beijing showed wide distribution characteristics. Raw meat products were the main source of Salmonella isolates from food.