2012年十堰市肠道细菌分离鉴定能力考核结果分析

Analysis of isolation and identification ability of Enteric bacteria in Shiyan city in 2012

目的加强病原微生物实验室能力建设,提高对肠道传染病疫情检测水平。方法用细菌培养法对考核盲样的目标菌(分别为霍乱弧菌、志贺氏菌、沙门氏菌、大肠埃希氏菌)进行增菌、分纯,依据细菌生长特性、革兰染色形态、生化试验和血清学试验结果综合判断。结果考核成绩满意。5份考核盲样均加有干扰菌株,其中有一考核盲样为混合菌。结论盲样中加有干扰菌株和混合菌株,增加了考核难度,能及时发现平时工作中存在的问题,促进疾病控制机构卫生检测水平的不断提高。

Objective To strengthen the ability construction of pathogenic microorganism lab and improve detection level of en teric infectious disease outbreak. Methods The target bacteria for blind samples check including Vibrio cholerae, Shigella, Salmonella, Escherichia coli were conducted enrichment and purification by bacteria culture method, then the test results were judged by bacterium growth characteristics, Gram＇s staining shape, biochemistry experiment and serologic test. Results The check results was satisfactory. Five unknown samples all contained the interfering strains, and one of the blind samples had mixed bacteria. Conclusion Interfering bacteria and mixed bacteria in blind samples have increased the examination difficulty. Bhnd samples examination can contribute to timely find the existing problems in daily work and improve the hygiene detection level of diseases control institutions.