脓毒症与IRAKM基因多态性的相关性

The correlation between sepsis and IRAKM genetic polymorphisms

目的探讨人IRAKM+22148位点的基因多态性与脓毒症易感性和预后的关系。方法采用聚合酶链反应(PCR)-限制性片段长度多态性分析法(RFLP)进行基因多态性分析。比较病例组与对照组基因型频率及等位基因频率分布差异,分析IRAKM+22148位点基因多态性与脓毒症易感性的关系。计算等位基因OR值和95%可信区间评价其相对患病风险。比较脓毒症患者存活组与死亡组的基因型频率及等位基因频率,分析IRAKM+22148位点基因多态性是否与脓毒症预后相关。结果病例组与对照组比较基因型频率分布差异无统计学意义(P>0.05)。而两组间G等位基因频率差异具有统计学意义(P<0.05)。OR=2.24(95%CI 1.25-4.00)。脓毒症患者存活组与死亡组比较,基因型频率和G等位基因频率,差别皆无统计学意义(P>0.05)结论 IRAKM+22148位点G等位基因可能是脓毒症易感基因。未发现脓毒症病死率与IRAKM+22148位点基因变异相关。

Objective To investigate the association between IRAK M ＋ 22148 genetic polymorphism and the susceptibility and the prognosis of sepsis. Methods The gene polymorphisms were assessed by polymerase chain reaction（PCR） and the re striction fragment length polymorphisims （RFLP）. Genotype and allele distribution were compared to assess the association be tween IRAKM polymorphism and the susceptibility of sepsis. Odds ratios （OR） and 95% confidence intervals （CI） of allele were calculated to assess the relative disease risk conferred. Mortality rates of different genotypes were compared to assess the association between IRAKM polymorphism and the prognosis of sepsis. Results No significant statistical differences of geno type frequency were found in sepsis group and the controls （P 〉 0.05 ）. However, the G allele was more frequent in sepsis group as compared to the controls （P 〈 0.05, OR = 2.24, 95 % CI 1.25 4.00）. No significant statistical differences of genotypes and G allele were found in survival group and death group （P 〉 0.05 ）. Conclusion G allele may be the susceptive gene of sepsis. No relationship was found between gene variation of IRAKM ＋ 22148 site and mortality rates of sepsis.