三种方法检测尿液白细胞的对比分析

Comparative analysis of three methods for urine leukocyte detection

目的探讨Sysmex UF1000-i全自动尿有形成分分析仪、AX-4280尿干化学分析仪及显微镜检三种方法检测尿液白细胞的差异性。方法对968例患者尿液分别用上述三种方法进行检测,分析比较尿白细胞检测结果的差异性。结果白细胞检测UF-1000i及尿干化学阳性率分别为27.07%和15.29%,与显微镜镜检阳性率18.08%比较,前者差异有统计学意义(P<0.05,χ2值为22.37),而后者无统计学差异;UF-1000i法与显微镜镜检的符合率为90.81%,尿干化学法与显微镜镜检的符合率为91.84%;以镜检结果为参考标准,UF1000-i检测88例假阳性结果,主要是小圆上皮细胞;尿干化学检测53例假阴性结果,主要是淋巴细胞,26例假阳性结果,主要是鳞状上皮细胞。结论在进行尿液分析时三种方法应联合应用,以提高检测的准确性及尿液分析的质量。

Objective To discuss the difference of Sysmex UF1000 -i automatic urinalysis analyzer, AX- 4280 urine dry chemistry analyzer and microscopy for detection of urine leukocyte. Methods Urine samples were collected from 968 patients for leukocyte detection by the three methods, then the results were compared to analyze the difference. Results The positive rates of UF - 1000i, urine dry chemistry analyzer and microscopy were 27.07%, 15.29% and 18.08% respectively. The difference had statistical insignificance between UF - 1000i and microscopy（P 〈 0.05, X2 = 22.37）, and the coincidence rate was 90.81%. While there was no statistical significance between urine dry chemistry analyzer and microscopy, and the coinci- dence rate was 91.84%. With microscopy result as reference standard, there were 88 false - positive samples by UF - 1000i, mainly small round epithelial cells. But by urine dry chemistry analyzer, there were 53 false - negative samples in lymphocyte cells and 26 false - positive samples in squamous cells. Conclusion Three methods should be combined in urine analysis to improve the accuracy and quality of detection.