2011年-2012年龙岩市手足口病标本病毒检测结果分析

Analysis of HFMD detection results in Longyan in 2011 and 2012

目的通过对2011年-2012年龙岩市临床诊断病例进行病原学检测,了解手足口病的感染情况和病原学特征,为手足口病的诊断提供科学依据。方法采用荧光RT-PCR方法对2011年-2012年龙岩市手足口病患者及其密切接触者848份标本进行肠道病毒及EV71、COXA16的检测。结果肠道病毒总阳性率为64.86%,其中EV71阳性率为23.94%,COXA16阳性率为19.70%,其他肠道病毒阳性率为21.23%;5岁以下儿童是主要感染人群,占实验室检测人数的91.04%,阳性率达65.80%。结论龙岩市2011年-2012年手足口病以EV71为主要病原,同时应加强其他肠道病毒的监测,做好预防控制措施。

Objective To detect the pathogens in clinical patients in Longyan in 2011 and 2012, understand the infection status and etiological characteristics of HFMD, so as to provide scientific evidence for HFMD diagnosis. Methods Real - time fluo- rescent PCR was used for enterovirus nucleic acid detection in 848 samples （HFMD patients and close contacts） collected in Longyan from 2011 to 2012. Results The total positive rate of enterovirus was 64.86% , including EV71 （23.94%）, COX- A16 （19.70%） and other intestinal virus（21.23% ）. Children under 5 years old were the major infection population, account- ing for 91.04% in all 848 samples, and the positive rates was 65.80%. Conclusion EV71 was the main pathogen of HFMD in Longyan from 2011 to 2012. Meanwhile, the monitoring of other intestinal virus also should be strengthened to prevent and control the HFMD infection.