基于代谢组学研究姜制对黄连药性的影响

Effect of Coptidis Rhizoma processed with ginger juice on its property based on metabonomics

目的探讨黄连姜制前后寒热药性的差异。方法分别制备生黄连、姜黄连水煎液,每天ig给予大鼠1次,连续给药29 d,于给药后不同时间点采集血样,进行HPLC-MS/MS分析,主成分分析(PCA)法处理实验数据,分析生黄连和姜制黄连在血浆中的代谢物的差异。结果给药第29天生黄连组和姜黄连组大鼠血样中内源性物质的代谢状态差异最大。通过查找内源性生物标记物发现,与生黄连组相比,姜黄连组大鼠血样中代谢物中涉及到氨基酸能量代谢的标记物的量增加,提示姜制黄连组氨基酸能量代谢强于生黄连,二者寒热药性存在差异。结论通过大鼠代谢物生物效应可以考察生黄连与姜黄连药性的差异,且结果与传统的"姜制黄连以热制寒,缓和黄连苦寒之性"的炮制理论相一致。代谢物组学可用于探讨炮制对药物药性影响的研究。

Objective To explore the difference of hot and cold property of Coptidis Rhizoma （CR） before and after being processed with ginger juice. Methods To feed rats for 29 d with the decoction of un-processed CR （UPCR） and ginger juice-processed CR （GPCR）, the rats＇ blood was collected at different administration time, the data by HPLC-MS/MS analysis were processed with PCA to determine the metabolites difference between the treatment groups. Results On the day 29, the metabolic status of endogenous substances in rats＇ blood showed the biggest difference between the UPCR and GPCR groups. Through checking the endogenous biomarkers of the rats in each group, the content of markers related to amino acid energy metabolism of rats in the GPCR group was higher than that in the UPCR group, indicating the energy metabolism of the GPCR was stronger than that of the UPCR, which showed the differences in cold and hot property between the two drugs. Conclusion The results show that the differences in potency of the UPCR and GPCR can be reflected by rats＇ different biological effects. The results are consistent with the traditional processing theory in Chinese materia medica that the cold property of CR could be moderated to be colder by processing with assistant materials with hot property （ginger juice）. The work indicates that the metabonomic method is a valuable tool in the research of processing effect on properties of Chinese medicinal substances. The results are helpful to clarify the mechanism of processing CR with ginger juice in the future.