摘要
Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes （glutathione reductase, GR; glutathione S-tramsferase, GST） and soluble sugar content of cucumber fruit were signiifcantly increased, but malonaldehyde （MDA） content was signiifcantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis （2-DE） protein proifles of control and propamocarb-treated fruits, and identiifed 18 differentially expressed （13 up-regulated and 5 down-regulated） proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-lfight mass spectrometry （MALDI-TOF-MS）. The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction （qRT-PCR） was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.
Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes （glutathione reductase, GR; glutathione S-tramsferase, GST） and soluble sugar content of cucumber fruit were signiifcantly increased, but malonaldehyde （MDA） content was signiifcantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis （2-DE） protein proifles of control and propamocarb-treated fruits, and identiifed 18 differentially expressed （13 up-regulated and 5 down-regulated） proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-lfight mass spectrometry （MALDI-TOF-MS）. The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction （qRT-PCR） was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.
基金
supported by the National Natural Science Foundation of China(21372158)
