乳鼠心肌成纤维细胞MyoDcDNA转染后的形态变化

Morphological changes of myocardial fibroblasts of neonatal rat following transfection with MyoD cDNA in vitro

目的 探讨运用MyoD基因转染使乳鼠心肌间质细胞转化为肌细胞的可能性。方法 MyoD基因是具有碱性螺旋 环 螺旋结构的生肌因子之一 ,用脂质体介导法将MyoDcDNA导入培养的乳鼠心肌间质细胞 (主要是成纤维细胞 )。结果 心脏成纤维细胞在导入MyoDcDNA 5d后可见 :①部分细胞形态发生改变 ,类似骨骼肌细胞 ,胞体伸展 ,胞浆颗粒增多 ;②经原位杂交证实 :1.8%～ 10 .9%的细胞有下游生肌分化标志即骨骼肌肌球蛋白重链mRNA的表达。结论 表明心肌成纤维细胞可转化为具有潜在功能的类骨骼肌细胞 。

Objective To evaluate the possibility of converting cultured myocardial mesenchymal cells (mostly cardiac fibroblasts) of neonatal rat to skeletal muscle cells by the transfection with MyoD gene, one of the basic helix loop helix myogenic factors. Methods The plasmid MyoD cDNA was transferred into cultured myocardial mesenchymal cells of neonatal rat with liposome mediated method. Results Five days after the transfection, the myocardial fibroblasts showed that: ①Some of them were morphologically similar to skeletal muscle cells, cell body elongated and cytoplasmic particles increased; ②The expression of downstream myogenic differentiation marker(myosin heavy chain mRNA) was observed, in 1.8% to 10.9% of the transfected cells with in situ hybridization with specific MyoD cDNA probe. Conclusion The myocardial fibroblasts can be converted to “skeletal muscle like” cell with potential function. These results suggest that it is possible to restore cardiac function via molecular cardiomyoplasty.