纤状蛇纹石石棉对兔肺泡巨噬细胞影响的体外研究

Study on the effect of chrysotile asbestos on rabbit pulmonary alveolar macrophages injury in vitro

目的 探讨纤状蛇纹石石棉致肺泡巨噬细胞受损的机制。方法 采用体外细胞培养技术 ,以兔肺泡巨噬细胞死亡率、乳酸脱氢酶 (LDH)、超氧化物歧化酶 (SOD)活力及丙二醛 (MDA)含量变化来表示不同剂量、不同培养时间的纤状蛇纹石石棉对巨噬细胞的毒性。结果 纤状蛇纹石石棉对肺泡巨噬细胞的毒性存在着剂量 效应关系、时间 效应关系 ;12 5 μg/ml培养 18h后 ,细胞死亡率及LDH增加 ,5 0 0 μg/ml培养 5h后 ,细胞死亡率、LDH、MDA均明显增加 ,而SOD活力明显降低。随粉尘剂量的增加及培养时间的延长 ,细胞毒性也增加。

Objective To study the mechanism of damages of pulmonary alveolar macrophages caused by chrysotile asbestos. Methods Rabbit pulmonary alveolar macrophages were cultured in vitro . The cell death rate, the malandialdehyde (MDA), lactate dehydrogenate (LDH) and superoxide dismitase(SOD) contents in the cultured cells were determined at different incubation time and different dose of chrysotile asbestos for the cytotoxicity of the material. Results The cytotoxicity of chrysotile asbestos was in a dose and incubation time dependent manner. After 18 h incubation and at the dose of 250 μg/ml, the cell death rate and LDH content were increased significantly. In 5 h incubation and 500 μg/ml dose, the cell death rate, LDH and MDA all had a significant increase. Conclusion The cytotoxicity of chrysotile asdestos on pulmonary alveolar macrophages maybe related to the surface free radicals of chrysotile asbedtos.