三七总皂苷联合牛磺酸对脑缺血再灌注损伤模型大鼠的保护作用

Protective Effects of Panax notoginseng Total Saponins Combined with Sulfuric Acid Against Cerebral Isch-emia-reperfusion Injury Model Rats

目的:研究三七总皂苷联合牛磺酸对脑缺血再灌注损伤模型大鼠的保护作用。方法:复制大鼠中动脉缺血再灌注模型。90只SD大鼠随机分为正常对照(等容生理盐水)组、假手术(等容生理盐水)组、模型(等容生理盐水)组、尼莫地平(40 mg/kg)组、三七总皂苷(240 mg/kg)组、牛磺酸(60 mg/kg)组与联合用药高、中、低剂量(三七总皂苷480、240、120 mg/kg+牛磺酸120、60、30 mg/kg)组。术前1 h及再灌注开始1 h后分别灌胃给药1次。测定大鼠神经缺损评分、脑梗死面积、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,观察大鼠脑组织形态学。结果:与假手术组比较,模型组大鼠神经缺损评分显著增加,脑梗死面积显著增加,SOD活性显著减弱,MDA含量显著增加,大鼠大脑形态严重受损。与模型组比较,三七总皂苷组、牛磺酸组与联合用药高、中剂量组大鼠脑梗死面积显著减少,SOD活性显著增强,MDA含量显著减少,大鼠大脑形态学有一定改善,且联合用药高、中剂量组以上指标均好于三七总皂苷组、牛磺酸组。结论:三七总皂苷与牛磺酸联合用药可在一定程度缓解大鼠脑缺血再灌注引起的损伤,其机制可能与降低神经缺损评分、改善大脑形态学与调节生化指标有关。

OBJECTIVE： To study the protective effects of Panax notoginseng total saponins combined with sulfuric acid against cerebral ischemia-reperfusion injury in rats. METHODS： Arteriae cerebri media ischemia-reperfusion injury model was in- duced. 60 SD rats were randomly divided into normal control group （constant volume of normal saline）, sham operation group （constant volume of normal saline）, model group （constant volume of normal saline）, nimodipine group （40 mg/kg）,P, notoginseng total saponins group（240 mg/kg）, ethylamine sulfonic acid group （60 mg/kg）, drug combination high-dose, medium-dose and low-dose groups （P. notoginseng total saponins 480, 240, 120 mg/kg＋ethylamine sulfonic acid 120, 60, 30 mg/kg）. They were given medicines intragastrically lh before operation and lh after reperfusion. The nerve defect score, cerebral infarction area, SOD activity and MDA content were determined, and the morphology of the brain was observed. RESULTS： Compared with sham opera- tion group, neurological deficit score, cerebral infarction area and MDA content increased significantly in model group, while SOD activity decreased significantly; cerebral morphology of rats had badly damaged. Compared with model group, cerebral infarction area and MDA content decreased significantly in P. notoginseng total saponins group, ethylamine sulfonic acid group, drug combi- nation high-dose and medium-dose groups, while SOD activity increased significantly; cerebral morphology of rats had certain im- provement. Above index of drug combination high-dose and medium-dose groups were better than those of P. notoginseng total sapo- nins group and ethylamine sulfonic acid group. CONCLUSIONS：P notoginseng total saponins combined with ethylamine sulfonic acid can alleviate cerebral ischemia-reperfusion injury to some extent, and its mechanism may be related to reduction of nerve defect score and improvement of cerebral morphology and adjustment of biochemical indexes.