HPLC法测定香椿叶中山柰酚-3-O-鼠李糖苷的含量

Content Determination of Kaempferol-3-O-rhamnoside in Toona sinensis by HPLC

目的：建立测定香椿叶中山柰酚-3-O-鼠李糖苷含量的方法，为其质量控制提供参考。方法：采用高效液相色谱法。色谱柱为Thermo Hypersil ODS（150mm×4．6mm，5um），流动相为甲醇-水（55：45，V／V），流速为1．0ml／min，检测波长为254nm，柱温为35℃。结果：山柰酚-3-O-鼠李糖苷的质量浓度在5．14-51．40μg／ml范围内与其峰面积积分值呈良好的线性关系（r=0．9997）；精密度、重复性、稳定性试验的RSD〈2％；平均加样回收率为96．77％～103．02％，RSD为3．75％～4．64％（n=3）。结论：该方法准确、重复性和稳定性好，可用于香椿叶中山柰酚．3．D．鼠李糖苷的含量测定。

OBJECTIVE： To establish a method for the content determination of kaempferol-3-O-rhamnoside in Toona sinensis, and to provide reference for quality control of it. METHODS： HPLC method was adopted. The determination was performed on Thermo Hypersil ODS （150 mm×4.6 mm, 5 μm） column with mobile phase composed of methanol-water （55：45, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 254 nm and column temperature was 35 %. RESULTS：The linear range of kaempferol-3-O-rhamnoside were 5.14-51.40μg/ml（r=0.999 7）. RSDs of precision, reproducibility and stability tests were all lower than 2%, and average recoveries were from 96.77% to 103.02% （n=3）. RSDs were 3.75%-4.64% （n=3）. CONCLUSIONS： The method is accurate, reproducible and stable. It can be used for the content determination of kaempferol-3-O-rhamnoside in T. sinensis.