摘要
目的:通过研究转化生长因子-β1(Transforming Growth Factor-β1,TGF-β1)在溃疡性结肠炎大鼠模型中的表达情况,探索TGF-β1在溃疡性结肠炎发病过程中的作用。方法:70只SD大鼠按体重随机分为空白对照组30只和模型组40只,RNBS灌肠法复制大鼠溃疡性结肠炎模型,分别在第8、29、50天每组处死10只大鼠,取结肠组织,采用HE染色,光镜下观察组织形态学的变化,免疫组化测定结肠组织TGF-β1的表达。结果:光镜下模型组大鼠结肠黏膜上皮细胞萎缩、脱落,组织结构破坏不完整,黏膜上皮炎性细胞浸润,部分可见黏膜表面糜烂、小溃疡形成,纤维结缔组织增生。模型组大鼠结肠组织TGF-β1表达在第8、29、50天均明显高于空白对照组(P<0.05)。结论:转化生长因子-β1在溃疡性结肠炎的发生发展过程中起着重要的作用,TGF-β1的表达与疾病的严重程度密切相关。
Objective： To explore the function of Transforming Growth Factor -β1 （TGF-β1） on ulcerative colitis in rats model, researching the expression of TGF-131 of ulcerative colitis rats model. Methods：To replicate ulcerative colitis model of rats which were randomly divided into blank control group and model group according to the weight. There were 30 rats in blank group and 40 rats in model group. We executed 10 rats each time to get the colon respectively on the 8th ,29th ,50th day in each group, using HE staining and light microscope to observe histomorphology change, immunohistochemical determination of colon tissue, expression of TGF-β1, and carries on the quantitative analysis using image analyzer. Results： The TGF-β1 of the model group on the 8th,29th,50th day were significantly higher than that of control group. And most of colon tissue had obvious deformation and necrosis. Conclusion： TGF-β1 takes important function in the onset and development of ulcerative colitis. It is closely related with the severity of disease and can provide more scientific basis for the diagnosis and treatment of ulcerative colitis.
出处
《实用中西医结合临床》
2013年第7期1-3,共3页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
国家重点基础研究发展计划("973"计划)资助项目(NO:2009CB522706)
