鼻息肉组织中细胞增殖与凋亡相关基因蛋白的表达及意义

Expression of cell proliferation and apoptosis gene associated protein on nasal polyps and its significance

目的 探讨以嗜酸粒细胞浸润为主要病理特征的鼻息肉组织细胞增殖和凋亡的规律 ,以加深对鼻息肉病理机制的了解。方法 以免疫组化染色法对 2 6例 (2 6侧 )鼻息肉和 14例 (14侧 )正常下鼻甲组织行增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)及与细胞凋亡相关基因Bax、抑凋亡基因Bcl 2检测。结果 ①鼻息肉粘膜上皮PCNA、Bcl 2和Bax阳性表达均显著高于下鼻甲组织 ,但Bcl 2与Bax阳性表达百分率的比值与下鼻甲组织无明显差异 ,表明鼻息肉上皮细胞凋亡趋势较为明显 ;②鼻息肉组织中腺体、嗜酸粒细胞Bcl 2的阳性表达显著高于Bax ,而下鼻甲组织中二者则差异无显著性。结论 鼻息肉上皮细胞有显著的增殖特性 ;凋亡基因Bax和抑凋亡基因Bcl 2表达失衡使细胞凋亡受抑制是鼻息肉组织中嗜酸粒细胞数增多的重要因素之一。

Objective To further understand the pathogenic mechanism of nasal polyps, namely the cells proliferation and apoptosis in nasal polyps tissue. Methods The proliferation cell nuclear antigen (PCNA), apoptosis associated gene protein (Bcl-2,Bax) were determinated in 26 tissue samples of nasal polyps and 14 controls from normal inferior turbinates respectively. Results (1) The positive expression e of PCNA, Bcl-2 and Bax were significantly higher in epithelium of nasal polyps than the controls, and the expression rate of Bcl-2/Bax, however, was tend equipoise. (2) The expression of Bcl-2 were significantly stronger than Bax in glands, eosinophils of nasal polyps tissue, and there were not significante differences in inferior turbinates. Conclusion There is strongly proliferation activity in the epithelium of nasal polyps, and expression imbalance of Bcl-2/Bax may be one of the important factors of eosinophilia in nasal polyps tissue.