SPARC在肺纤维化鼠肺组织的动态表达及法舒地尔的干预效应

Change of SPARC expression and intervention effect of fasudil on the expression of SPARC in mice pulmonary fibrosis tissue

目的研究富含半胱氨酸的酸性分泌蛋白（sPARc）在博莱霉素（BLM）诱导小鼠肺纤维化肺组织中的动态表达及法舒地尔的干预效应，探讨SPARC在肺纤维化中的作用机制。方法将108只小鼠分成正常对照组（Cont组），BI。M组，法舒地尔（FAs）组。分别给予等量的0．9％氯化钠注射液0．04ml和BLM（3．5mg／kg）一次性气管内灌注，FAS组在BLM一次性气管内灌注后给予及FAS15mg／kg腹腔注射。各组分别于第3天、第7天、第14天和第28天各处死8只小鼠，取肺组织做病理切片，用苏木精-伊红（HE）染色观察小鼠肺组织的病理形态改变；TuNEL细胞凋亡原位检测BLM组与Cont组肺组织细胞凋亡水平；Westernblot检测三组肺组织SPARC及Rho激酶-1（Rock-1）蛋白的定量表达，并计算SPARC蛋白与凋亡指数的相关系数。结果小鼠108只进入结果分析，模型组小鼠死亡8只。①HE染色：BI。M组肺组织逐渐由肺泡炎进展为纤维化。②TUNEI。细胞凋亡原位检测：与Cont组相比，BI。M组肺泡上皮细胞凋亡指数呈进行性上升。③Westernblot结果显示：BLM组SPARC、Rock-1的表达显著增高。FAS组SPARC、Rock-1的表达逐步下降。BLM组SPARC蛋白与凋亡指数相关系数表明SPARC蛋白表达与肺泡上皮细胞凋亡呈正相关。结论在小鼠肺纤维化进程中存在SPARC过表达，过表达的SPARC蛋白对肺纤维化可能起促进作用。抑制Rho／Rho激酶信号通路可减少sPARC蛋白表达，在延缓肺纤维化进程中可能起重要作用。

Objective To observe the changes of SPARC expression and intervention effect of fasudil in experimental mice induced by bleomycin, so as to discuss the action mechanism of SPARC in pulmonary fibrosis. Methods According to the random number table, all 108 mice were randomly divided into control group （ n =36）, model group （ n =36） and fasudil group （ n z36）. The three groups were injected with equal 0.9% sodium chloride injection and BLM （3.5 mg/kg） by disposable syringe firstly. The FAS group was injected with intraperitoneal FAS 15 mg/kg injection after BLM injection. Eight mice of each group were killed respectively at the 3rd,7th, 14th,28th day and the lung tissues were made into pathological sections. Histological changes were observed by HE staining and the apoptosis level of lung tissue of BLM group and Cont group were tested by TUNEL, while western blot were used to detect the expression of SPARC and Rock-1 of the three groups. Moreover, the correlation coefficient between SPARC and apoptosis index was calculated. Results One hundred and eight mice were involved in the result analysis and eight mice of the model group died. （1）HE staining： the BLM lung tissue gradually changed from pulmonary alveolitis into fibrosis was observed. （2）The TUNEL.. compared to those in the Cont group, the apoptosis index of lung cells in the BLM group was increasing progressively. （3）The result of Western blot presented that the expression of SPARC and Rock-1 of BLM increased ＇significantly. Compared to the BLM group, the level of SPARC and Rock-1 expression were decreased gradually in the FAS group. The correlation coefficient between SPARC and AI in the BLM group suggests that there exists positive correlation between AI and the expression of SPARC in the model group. Conclusions There exists abnormal expression of SPARC in the progression of mice pulmonary fibrosis. The abnormal expression of SPARC may play a driving role in the pulmonary fibrosis. The dynamic expression of SPARC may have a negative effect on the cell cycle of alveolar epithelial cells. To suspend the progression of PF, it might be very important to restrain the Rho/Rho kinase signal pathway.