二氟甲基鸟氨酸对K562细胞生长特性的影响及其与端粒酶活性的相关性

Effect of DFMO on the Growth of K562 Cells and Its Association with Telomerase Activity

目的 :研究二氟甲基鸟氨酸 (DFMO)对K5 6 2细胞生长特性、调亡及端粒酶活性的影响。方法 :采用细胞计数、形态观察、FCM分析及DNA电泳分别检测DFMO处理的细胞生长速率、周期公布及细胞凋亡。按TRAP法对细胞端粒酶活性进行检测。结果 :DFMO处理的细胞生长速率明显减曼 ;G1期细胞增多而S期细胞减少 ;细胞表现凋亡诱导 ,且其端粒酶活性受到抑制。结论 :DFMO引起的K5 6 2细胞生长抑制及凋亡诱导与端粒酶活性抑制有关。端粒酶的失活是抑制多胺生物合成的抗癌分子机制重要事件之一。

Objective: To investigate the effects of DFMO on the growth characteristics,apoptosis and activity of telomerase of K562 cells.Methods:The growth rate,phase distribution of cell cycel and apoptosis of treated cells with DFMO were detected by cell cuout,morphological assay,FCM analysis and DNA electrophoresis,respectively.The telomerase activiety was examined by telomeric repeat amplification protocol(TRAP).Results: In K562 cells treated with DFMO,the growth rate was markedly retarded.The incrases in G 1-phase cells and decrease in s-phase population and induction of apoptosis were observed.The activity of telomerase of treated cells was inhibited.Conclusion: The growth inhibition and apoptosis induction of K562 cells treated by DFMO were associated with suppressed telomerase activity.It is suggested that inactivation of telomerase in cancer cells conld be one of important events in antitumor molecular mechanism of inhibition of polyamine biosynthesis.