更昔洛韦对慢性粒细胞白血病细胞生长和分化影响及其机制研究

Effect of Gancyclovir on Proliferation and Differentiation of Chronic Myeloid Leukemia Cells and Research of Its Mechanism

目的 :探讨更昔洛韦 (GCV)对人白血病细胞的抗增殖和诱导分化作用。方法 :GCV加入K5 6 2细胞培养 4d ,测定其活细胞数目、克隆形成率、联苯胺染色阳性率 ,观察光镜形态、组织化学染色 ,并进行流式细胞分析、端粒酶检测。结果 :在GCV的作用下 ,K5 6 2细胞的增殖受抑 ,生长分数降低 ,并且向具有合成血红蛋白能力的细胞分化。结论 :GCV对K5 6 2细胞具有抑制增殖和诱导分化作用 ,该结果为慢性粒细胞白血病 (CML)的治疗探索新的途径。

Objective: To explore whether gancyclovir (GCV) can inhibit the proliferation and induce the erythro differentiation of the K562 human myeloid leukemia cell line. Methods: K562 cells were cultured with GCV for 4 days to detect cellular changes cloning efficiency, benzidine positive rate, flow cytometry analysis, and telomerase activity. Results: When K562 cells grew in the medium containing GCV, the cellular growth and division were gradually suppressed, growth fracture decreased and further differentiation towards the cell producing hemoglobins was found. Conclusion: GCV can inhibit proliferation and induce erythro differentiation of K562 cells.