人参皂甙与IL-2协同诱导PBMC对恶性脑胶质瘤细胞杀伤活性研究

Cytotoxicity of GS and IL-2 Activated PBMC to Malignant Glioma Cells

目的 :探索提高恶性脑胶质瘤治疗效果的新途径。方法 :用人参皂甙 (ginsenodide ,GS)与白细胞介素 2 (IL - 2 )协同诱导人外周血单个核细胞 (PBMC)制成GS -LAK细胞 ,用MTT法测定其对恶性脑胶质瘤细胞 (BT32 5 )的杀伤活性 ,并与LAK细胞相比较。结果 :效应细胞GS -LAK在增殖数量和杀伤恶性脑胶质瘤细胞活性等方面均优于LAK细胞 ,且IL - 2用量减少。结论 :采用GS -LAK细胞 ,可提高效应细胞的数量和活性 ,为恶性脑胶质瘤的免疫治疗打下了理论基础。

Objective: To investigate a new method for improving the therapeutic effect on malignant glioma. Methods: A new type of killer cells, named GS-LAK, was induced by means of costimulating the peripheral ginsenoside(GS) and in-terleukin-2 (IL-2). Comparing with control group-LAK cells, cytotoxicity of GS-LAK cells against malignant glioma cells(BT325) was examined with MTT method. Results: It showed that GS-LAK cells exhibited some advantages over LAK cells in proliferation, cytotoxicity, as well as the utilizing of IL-2. Conclusion: The application of GS-LAK cells might open a new prospect to clinical therapeutic approach to malignant glioma.