3,7-二硝基二苯并溴五环盐诱导K562肿瘤细胞凋亡作用研究

Apoptosis of K562 Cells Induced by 3,7-Dinitrodibenzobromonium Salt

目的 :研究 3,7-二硝基二苯并溴五环盐对人慢性粒细胞红白血病细胞株K5 6 2增殖的影响及其凋亡机理。方法 :采用台盼蓝拒染法 ,MTT比色法及克隆形成率等方法 ,并用光学、荧光及电子显微镜观察照相 ,流式细胞术等方法分析DNA断裂的数值。结果 :cBr对K5 6 2具有杀伤作用 ,可明显抑制K5 6 2细胞增殖 ,呈现时间和剂量效应 :克隆形成率测得IC50 为 0 .4 97mmol/L ,流式细胞术测定亡率为 70 .34 % ;荧光显微镜和电镜观察到明显凋亡特征。结论 :cBr抑制肿瘤增殖是通过诱导肿瘤细胞凋亡实现的。

Objective: To investigate the antitumor activities and apoptosis mechanisms of 3,7-dinitrodibenzobromonium salt (cBr) on human chronic myelogenous leukemia K562 cell line in vitro. Methods: The antitumor activities of cBr were tested by Trypanblau exclusion test ,MTT coloretric assay and clonal forming test,and the mechanisms were studied by morphological analysis(microscopy,fluorescencemicroscopy,electron microscopy)and flow cytometry analysis.Results: cBr could inhibit of the proliferation K562 cells and kill them significantly.The effects varied with different time and doses,and IC 50 of cell colony forming rate was 0.497 mmol/L.Apoptosis rate tested by flow cytometry was 70.34%,and apoptotic characteristics could be found by microscopy and electron microscopy. Conclusions: cBr could inhibit K562 cell proliferation greatly,and could induce K562 cells into apoptosis.