流式细胞术Ki67/DNA双标记法在实体肿瘤中的应用

Clinical application of Ki67/DNA bivariate flow cytometry on solid tumor

目的探讨流式细胞术双标记法检测恶性实体肿瘤细胞ＤＮＡ含量、细胞周期和Ｋｉ６７表达和组织学分级之间的关系。方法利用流式细胞术Ｋｉ６７／ＤＮＡ双标记法同时检测８７例新鲜恶性实体肿瘤的ＤＮＡ含量、细胞周期和增殖核抗原Ｋｉ６７的表达。结果异倍体发生率为４０．２％，其中高分化肿瘤为１１．ｌ％，中分化肿瘤为３７．５％，低分化肿瘤为 ４６． ３％。 Ｋｉ６７阳性细胞为 ０． ５％～８７％（１７． ３６％士１６． ６％）。 Ｓ期细胞百分比为 ０～２４％（５ ２８％±４． ８５％）。高分化肿瘤 Ｓ期细胞百分比及增殖核抗原 Ｋｉ６７的表达明显低于中分化和低分化肿瘤，统计学上有显著性差异（Ｐ＜０．０１）。中分化和低分化肿瘤之间则差异无显著性（Ｐ＞０．０５）。异倍体肿瘤Ｓ期细胞百分比高于二倍体肿瘤，差异有显著性（Ｐ＜０．０１），而Ｋｉ６７的表达在两者之间无显著性差异。结论流式细胞术Ｋｉ６７／ＤＮＡ双标记法可同时检测实体恶性肿瘤细胞的ＤＮＡ含量、细胞周期和增殖核抗原Ｋｉ６７的表达，并能进一步阐明这些参数与组织学分级的关系。方法简便、快速，有利于对肿瘤生物学特性的了解。

Objective To study the relationship between DNA content,S phase,Ki67 expression and histological grade of solid tumors by bivariate flow cytometry. Methods Ki67/DNA bivariate flow cytometry was used to detect DNA ploidy, cell cycle and Ki67 expression on 87 cases of malignant solid tumor. Results Aneuploidy was 40. 23% in all tumors,including 11. 1% on grade I, 37. 5% on grade Ⅱ and 46. 3% on grade Ⅲ. Ki67 possive cells varied from 0. 5%- 87% (17. 36 ± 16. 6% ). Cells on S phase ranged from 0-24% (5. 28±4. 85% ). S phase cells and Ki67 expression on grade I were significantly lower than those on grade Ⅱ and grade Ⅲ. No difference between those on grade Ⅱ and grade Ⅲ. S phase cells on aneuploid tumor were higher than on diploid tumor. There was no difference between aneuploid and diploid tumor on Ki67 expression. Conclusion Ki67/DNA bivariate flow cytometry can simultaneously detect DNA content, cell cycle and Ki67 expression on tumor cells. It is a valuable method for clinical application.