酶免疫法在粪便幽门螺杆菌诊断中的应用

The application of a EIA to diagnosis of helicobactor pylori in stool

目的 :探讨酶免疫测定法 (EIA)在粪便标本中幽门螺杆菌 (HP)诊断的实用价值。方法 :经胃镜组织学法和快速尿素酶试验 (RUT)确诊的 31例胃病患者的粪便标本 ,采用 EIA对其进行幽门螺杆菌抗原 (HPSA)的检测。结果 :以组织学法为标准 ,EIA的敏感性为 90 % ,特异性为 83.3% ,准确性为 95 % ,阳性预测值为 94.7% ,阴性预测值为71.4%。结论 :EIA测定 HPSA具有较高的敏感性和特异性 ,且方法简单、快速、取材方便 ,患者易于接受。对其感染疾病的诊断、疗效观察具有十分重要的意义 ,可望成为临床常规检测项目。

Objective:To study the practical values of EIA diagnosis in stool.Method:HPSA in feces speciment of 31 patients were detected by EIA after gastroscope histo logic methods and RUT make a definite diagnosis.Result:Hitologic techniques were regarded as a gold standard.The sensitivity and specificity of EIA were 90% and 83.3% respectively;the accuracy was 95%;the postive and negative values were 94.7% and 71.4% respectively.Conclusion:EIA for detecting HPSA in stool is a rapid,noninvasive both have high sensitivity and specificity.It has a very important significance for observation of diagnosis and curative effect of HP infection.