悬吊式人工晶状体植入术后组织病理学研究

Histopathological study after the three sutures suspended intraocular lens implantaton in rabbits

目的 观察一种新型人工晶状体植入兔眼的组织病理变化。方法 用 16只家兔建立动物模型 ,分别在术后 1周、2周、1月、2月摘除眼球 ,应用光镜、透射电镜、扫描电镜观察组织病理结构变化。结果 悬吊式人工晶状体植入兔眼后 ,炎症反应局限于缝线穿过的巩膜和睫状体部 ;聚丙烯缝线和眼部组织的连接不紧密 ,呈“袖套”样关系 ;人工晶状体镜片的支撑依靠 3根缝线 ,人工晶状体稳定性好。结论 悬吊式人工晶状体组织相容性好 。

ObjectiveTo observe the histopathological changes in the iris and ciliary body and a new intraocular lens(IOL) implantation in rabbits after lens extraction.MethodsIOLs were implanted into one eye of 16 rabbits.The three needles were passed through the corneoscleral limbal incision across the anterior chamber,posterior to the iris,through the ciliary body and out through the sclera at the 4,8 and 12 o'clock position.The three sutures were an interval of 120° and 0.5~1 mm behind limbus.The limbal incision was interrupted by nylon wire.The eyeballs were extracted at 1,2 weeks and 1,2 months postoperatively.The eyeballs were examined histopathologically by light microscope,scanning electron microscope,and transmission electron microscope.ResultsClear cornea and anterior uveitis,no hemorrhage were observed by slit lamp microscopy in one week.The inflammation changes were localized to iris,ciliary body,sclera.The growth of fibroblast cells and fibrous tissue on the surface of an IOL can be observed.The needle was situated in the ciliary sulcus in 17 cases,in ciliary processes in 19 cases,and in pars plana in 12 cases through ciliary body.The polypropylene(prolene) sutures weren’t intermixed with tissues.IOL was supported by the three sutures.IOL was very good in stability.ConclusionThe new IOL protocol showed good in histocompatibility.and the study can be considered a decent experimental reference for possible clinical applications.