碳纤维增强聚合物组织工程支架的制备及表征

The preparation and characterization of carbon fiber-reinforced polymer scaffolds for bone tissue engineering applications

以碳纤维(CF)作为增强材料,将CF有序排列于聚乳酸羟基乙酸(PLGA)多孔结构中,制备性能优良的CF/PLGA复合支架,并对其力学性能及细胞生物学性能进行表征。对增强体CF进行有序排列以提高支架的力学性能,扫描电子显微镜(SEM)观察CF/PLGA复合支架的微观形貌,可以看出CF在聚合物基体内部是呈有序结构并且二者结合情况良好。为了提高CF的生物相容性,利用对氨基苯甲酸对CF进行表面修饰,细胞生长在支架上的SEM照片反映了成纤维细胞对PLGA及CF/PLGA复合支架的黏附性能良好;通过细胞毒性测试,发现表面修饰的CF对细胞的生长没有负面作用,且在一定程度上促进了细胞的生长。研究结果表明,制备的CF/PLGA支架具有良好的力学性能和生物相容性,在骨组织工程支架的应用中具有一定的潜力。

The aim of this work is to improve mechanical properties by introducing carbon fibers （CFs） as the reinforcement into poly （lactic-co-glycolic） acid （PLGA）, resulting in a novel kind of scaffold for bone tissue engineering applications, meanwhile the mechanical properties and bioactivity were discussed. The arrangement of CFs was designed to improve the mechanical properties of scaffolds, and the Scan electron microscope （SEM） was used to characterize the morphology of aligned-CF/PLGA composite scaffolds. It was observed CFs ordered well in the polymer matrix and bonded with PLGA in a good condition. Para-aminobenzoic acid was used to enhance biocompatibility of CFs. SEM images of cells seeding on the scaffolds indicated cells could proliferate well on both pure PLGA scaffolds and the CF/PLGA composite scaffolds. The cytotoxicity test was carried out to characterize cells proliferation on the scaffolds. It was found surface treated-CFs had no adverse effect on cell growth but could promote cell growth to some extent. The results showed that the incorporation of CFs with PLGA is expected to possess good mechanical and biological properties and be potential for bone tissue engineering applications.