自动生化分析仪自动识别免疫比浊分析中的钩状效应

Automatic Check Method of Hook Effect in the Immunoassay with Automatic Clinical Chemistry Analysis?

目的 探讨全自动生化分析仪上识别钩状效应的方法 .方法 以IgG为检测对象 ,在HITACHI- 7170A型全自动生化分析仪上 ,抗原抗体反应 10分钟 ,利用定时监测 -时间反应进程曲线 ,分析其变化特征 ,选择钩状效应的识别参数 .结果 以反应时间 (读点 ) 2 0点的反应完成率 (ABS2 0 /ABS34 ) >0 90为限额参数 ,可自动识别钩状效应 .结论免疫比浊测定分析中的钩状效应 ,可通过正确的参数设置自动识别 .时间反应进程曲线是识别钩状效应的基础 ,反应率是识别钩状效应的有效方法 .

Objective To obtain automatic checck method of hook effect in the turbibimetric immunoassay with automatic clinical chemistry analysis.Methods With model 7170A automatic clinical chemistry analyzer,the method and value of automatic check of hook effect was obtained by assaying absorbance of immune complexes produced by which IgG of different concentratoin reacted with antibody for 10 minutes and traced the relationship between the quantity of antigen and absorbance,and by analyzing charactreistics of reaction monitor curve of immunofraction and tracing the relationship between the quantity of antigen and hook efect,and by calculating check value of the hook effect.Results The hook effect could be detected by automatic clinical analyzer if value,reaction rate of the 20th measurement point(ABS 20 /ABS 34 ),was lower than 0.9. Conclusions The check of hook effect in immunoreaction were performed by setting correctly limited value.Reaction monitor curve of immunoreaction was the basis of check of hook effect.The comparison of reaction was a kind of effective method of the check of hook effect.