实验性病毒性心肌炎纤维连接蛋白的免疫组化研究

An immunohistochemical study of the fibronectin in experimental viral myocarditis

探索轻度病毒性心肌炎的法医病理学诊断方法。以适量coxsackieB3病毒感染Balb/c小鼠 ,造成小鼠轻度病毒性心肌炎 ,对病鼠的心肌进行纤维连接蛋白的LSAB免疫组化染色。结果发现 ,心肌炎鼠心肌组织内Fn大量沉积 ,部分心肌细胞内Fn阳性。Fn -LSAB染色可显示病毒性心肌炎轻度的心肌损害 ,Fn沉积是心肌组织存在炎症性病理变化的可靠标志之一。

For the purpose of establishment of a diagnostic method of mild viral myocarditis,an experimental mild viral myocarditis model was induced in Balb/c murine by coxsackie virus B 3.The cardiac tissue secfion were stained by LSAB-immunohistochemical method with anti fibronectin(Fn) antibody. The results demonstrated that the Fn deposition was found in the myocardium of mice with myocarditis and the mild degeneration of cardiomyocytes could be identified by Fn LSAB immunohistochemical staining.It is suggested that the Fn deposition in the myocardium tissue is one of the reliable marks of myocardium inflammation.