饮酒对尿2-硫代噻唑烷-4-羧酸排泄的影响

Influence of drinking on urinary excretion of 2-thiothiazolidine-4-carboxylic acid

目的 观察饮酒对二硫化碳 (CS2 )接触者及非接触者尿 2 硫代噻唑烷 4 羧酸 (TTCA)排泄的影响。方法 (1)男性非接触CS2 志愿者 10人 ,一次饮用 38°白酒 15 0ml或 2 5 0ml,高效液相色谱法观察其尿TTCA排泄动态 ;(2 )CS2 作业男工 15 2人 ,非接触者 6 0人 ,分别收集班末尿和晨尿进行TTCA测定并进行问卷调查 ;同时进行个体空气采样和CS2 浓度气相色谱法测定。结果 非接触者一次饮白酒 15 0ml后 3h尿TTCA水平达峰值 ,12h后降至饮前水平 (餐前 0 .5h ,饮酒后 1、3、12h中位数分别为 0 .0 45、0 .0 6 8、0 .0 99、0 .0 46mg/gCr ,n =10 ) ;TTCA水平随饮白酒剂量的增加而增高 ,饮 0、15 0、2 5 0ml白酒者TTCA水平 (中位数 )分别为 0 .0 36、0 .0 6 4、0 .6 0 9mg/gCr(n =5 ,饮后 3h)。CS2 浓度为≤ 10 .0、10 .1～ 5 0 .0、>5 0 .0mg/m3 时 ,CS2 接触者TTCA有随CS2 浓度增高而上升的趋势 ;对照组中饮白酒和啤酒者TTCA水平似高于不饮者 ,而接触组TTCA水平则随饮酒指数的增加而呈降低趋势。结论 大量饮酒可影响尿TTCA水平 ,在进行CS2 生物监测时 ,应避免在大量饮酒后 12h内采集尿样 ,以避免饮酒对监测结果的干扰作用。

Objective To study the effect of drinking alcohol or beer on excretion characteristics of urinary 2 thiothiazolidine 4 carboxylic acid(TTCA) in peoples exposed to carbon disulfide(CS 2) and non exposure ones. Methods (1)Ten male non exposure volunteers drank 150 ml or 250 ml liquor(38°) within half an hour,and the excretion dynamic of urinary TTCA were detected with high performance liquid chromatograph.(2)Questionnarie and urine TTCA test were conducted in 152 male workers(end shift urine) exposed to CS 2 and 60 male control peoples(early morning urine).The ambient air at workplace was collected with active personal air monitoring and measured with a gas chromatograph. Results Urinary TTCA levels in non exposure volunteers reached a high peak after 3 hours of drinking 150 ml liquor,and 12 hours later returned to the levels before drinking,the median of TTCA before drinking,after drinking 1,3 and 12 hours were 0.045,0.068,0.099 and 0.046 mg/g Cr( n =10),respectively.TTCA contents were in accordance with the alcohol consumption,the median of TTCA after 3 hours of drinking 0,125 or 250 ml liquor were 0.036,0.064,0.609 mg/g Cr( n =5),respectively.There were increasing tendency of urine TTCA levels in workers exposed to different concentrations of CS 2(≤10.0 mg/m 3, 10.1～50.0 mg/m 3,>50.0 mg/m 3).When TTCA values were compared with drinking habit in various exposure situation,TTCA levels in people having drinking habit seemed higher(both liquor or beer) in non exposure group and lower in exposure group,especially in high concentration group(>50.0 mg/m 3). Conclusion Drinking large quantity of liquor may interfere the levels of urinary TTCA.Thus,in biomonitoring of CS 2 the suitable sampling time is important to avoid the bias estimation of CS 2 exposure.