红番3号加工番茄遗传转化再生体系的建立

A Regeneration System for Genetic Transformation of Processing Tomato Hongfan3

本研究以加工番茄红番3号子叶为外植体,对加工番茄再生体系无菌苗的苗龄、培养基激素的配比和培养基基质选择方面进行优化。结果表明:8～10 d的子叶为最佳的外植体。以MS为基本培养基,附加不同浓度IAA(0.05、0.10、0.15、0.20mg·L-1)分别与不同浓度6-BA(1.0、1.5、2.0、2.5mg·L-1)/ZT(0.1、0.5、1.5、2.0mg·L-1)的组合。经诱芽比较,在不同浓度6-BA/ZT与不同浓度IAA组合中,诱导出愈伤组织和不定芽分化最高的培养基为MS+ZT 0.5mg·L-1+IAA 0.1mg·L-1+植物凝胶2g·L-1。以1/2MS添加不同浓度IAA(0.05、0.10、0.15、0.20mg·L-1)进行生根比较,再生芽在1/2MS+IAA 0.1mg·L-1生根培养基上生根最好。

The cotyledon of processing tomato Hongfan3 was used as explants to establish a regeneration system for genetic transformation with different choices of seedling age, hormone composition and media. The results showed the best seedling-age were 8 -10 days. MS was used as basic medium, and different concentrations of IAA（0. 05,0.10, 0. 15,0.20mg·L^-1 ） combined with different concentrations of 6 - BA（ 1.0,1.5,2.0,2.5mg·L^-1 ） / ZT （0. 1,0.5,1.5, 2.0mg·L^-1 mg/ L） on bud induction. It showed that the best callus initiation and adventitious bud differentiation medium was MS ＋ ZT 0.5mg·L^-1 ＋ IAA 0. 1mg·L^-1 ＋ Phytagel 2g·L^-1 The best rooting medium was 1/2MS ＋ IAA 0. lmg·L^-1.