磁场处理紫苏种子的生物学效应

Biological Effects on Perilla frutescens Seed Treated with Magnetic Field

利用83.5mT(50A)磁场强度处理紫苏[Perilla frutescens(L.)Var.crispa]种子,研究不同处理时间对种子萌发及幼苗生长的影响,为探讨磁场对紫苏种子的作用机理提供依据。结果表明,83.5mT磁场处理紫苏种子后,种子发芽势、发芽率、真叶展开率、株高、根长、须根数以及根系活力都随着处理时间的延长呈先升高后降低的趋势;其中发芽势在2、3h时达到显著水平;发芽率以处理3h时达到最高,为97.3%;真叶展开率在1、2和3h三个处理条件下达到显著水平,且在3h时达到最大值;株高、根长及须根数均在3h时达到最大值;磁场处理3h时根系活力最强,达0.76TTF mg·(根·h)-1;POD活性及硝酸还原酶活性在2～3h时相对增幅较大;综合分析各指标说明83.5mT的磁场强度处理紫苏种子3h时,对刺激种子活力、促进种子萌发及幼苗生长效果最佳。

In this research, Perilla frutescens seeds were treated by the magnetic field of 83.5 mT with different processing time. At the same time, the seed germination, seedling growth, root activity and POD activity of seedlings were investigated to provide the theoretical basis of exploring the mechanism of plant seeds with magnetic treatment. The results showed that when Perilla seeds were treated by the magnetic field for 1 to 5 hours, seed germination rate, germination energy, true leaves expansion rate, plant height, root length, root number and root activity increased at first, then decreased following with the different treatment time. Among them germination energy and true leaves expansion rate reached the significant level under 3 hours treatment, germination rate was the highest at 3 hours and reached 97.3% , and plant height, root length and root number also reached maximum at 3 hours. When the magnetic treatment time was 3 hours, the root activity reached 0.76TTF mg/root＂ h while the POD and nitrate reductase activity increased obviously after the magnetic treatment for 2 hours. From this research, it was tested that the magnetic treatment of 83.5rot for 3 hours was the optimum treatment time to stimulate Perilla frutescens seed vigor, promote seed germination and seedling growth.