加压溶剂萃取法提取山楂中黄酮物质的工艺研究

The Study of Total Flavonoids from Crataegus Pinnatifide Bge with Pressurized Liquid Extraction

为优化加压溶剂萃取方法提取山楂总黄酮的工艺,采用单因素和正交试验,考察了提取温度,提取时间,循环次数,乙醇浓度对山楂中黄酮提取效果的影响。研究表明:加压溶剂萃取方法提取山楂黄酮的最佳工艺条件为提取温度80℃,提取时间5min,循环3次,乙醇浓度50%。在该条件下山楂黄酮最高提取率为4.99%,相对标准偏差为1.01%。加压溶剂萃取方法提取率明显高于超临界提取法和微波提取法,该方法所用试剂少,提取时间短,提取率高,用于黄酮等天然产物提取的前景广阔。

To obtain the optimum pressurized liquid extraction technology of flavones from Crataegus Prinnatifide bge, the single-factor extraction tests and orthogonal experiment were employed to optimize the technological conditions, including extraction temperature, extraction time, times of circulation and concentration of ethanol. The results indicated that the optimal extraction conditions were as follows： extraction temperature of 80℃ , extraction time of 3min, circulation of 3 times and solvent of 50% ethanol. Under the conditions, the highest extraction rate of flavones from Crataegus Prinnatifide bge was 4.99%. The relative standard deviation was 1.01%. The reproduction rate was high. The pressurized liquid extraction can extract more flavones from Crataegus pinnatifida Bge in shorter time with less solvent. It also shows a promising prospect for leaching the effective constituents from natural products by using pressurized liquid extraction.