水稻黄叶突变体功能叶衰老生理特性的研究

Research on Physiological Basis of Xantha Rice Mutant Functional Leaves Senescence

以水稻黄叶突变体及其野生型亲本为材料,研究了突变体叶剑叶衰老过程中若干生理特性的变化。结果表明,在花后5～25d,突变体剑叶的SPAD值、净光合速率和谷胱甘肽(GSH)含量、可溶性蛋白降低比其野生型亲本缓慢,抗坏血酸(ASA)和总抗坏血酸(total ASA)增加比野生型快,突变体酶促防御系统的保护酶超氧化物歧化酶(SOD)、细胞色素氧化酶(COX)和抗坏血酸过氧化物酶(ASPX)活性变化较小、相对稳定,清除衰老过程中产生的活性氧自由基的能力较强,突变体较其野生型亲本表现出延迟衰老的特性。

A xantha rice mutant and its wild type parent were used as materials to study the changes of some physiological characteristics during mutant flag leaves aging process. The results showed： during the 5th to 25th days after flowering, compared with its wild type parent, SPAD value, net photosynthetic rate （ Pn）, the content of glutathione （GSH） and soluble protein in the mutant flag leaves decreased slower; while ascorbic acid （ASA） and total aseorbic acid （total ASA） increased faster; the activities of enzymatic defense system protective enzymes superoxide dismutase （SOD） , cytoehrome oxidase （COX） and ascorbate peroxidase （ASPX） in mutant flag leaves have less change and they were relatively stable; they also have stronger ability to clear the active oxygen free radicals which generated in the aging process. Therefore, compared to its wild type parent, xantha rice mutant appeared an obvious features of delaying senescence.