猪瘟基因疫苗免疫小鼠外周血细胞免疫动态变化研究

Study on dynamic change of peripheral blood lymphocytes of mice vaccinated with classical swine fever virus DNA vaccines

应用FACS、MTT法对猪瘟基因疫苗pcDST、pcDSW免疫鼠外围血中CD+4 和CD+8淋巴细胞数、淋巴细胞的转化功能等动态变化进行了观察。结果 :pcDST、pcDSW猪瘟基因疫苗免疫小鼠后 ,小鼠的外周血淋巴细胞对LPS有强烈的反应性 ,CD+4 细胞数量增加 ,外周血淋巴细胞CD8+ 细胞数量在一段时间内增加 ,。CD+4 和CD+8细胞数量在免疫后 32天达到高峰后开始下降。表明pcDST、pcDSW质粒免疫小鼠后诱发免疫反应。

FACS, MTT were used for detecting the number of CD 4 + and CD 8 + T cells in splenocytes and peripheral blood lymphocytes of mice vaccinated with CSFV DNA vaccines, the mitogenic responsibility to ConA and LPS, The results demonstrated that The splenocytes and peripheral blood lymphocytes, from mice vaccinated with CSFV DNA vaccines, responsed to ConA and LPS 20 days after vaccinating, the number of CD 4 + and CD 8 + T cells reached the peak on 32th day after vaccinating..Summary: CSFV DNA vaccine can induce both humarol and cell mediated immunity;