杉木杂种群体分子框架遗传连锁图初报

The Construction of Molecular Linkage Frame Map in Chinese fir

利用随机扩增DNA多态性分子遗传标记—RAPD ,以杉木J0 和F1 1 两个亲本杂交形成的F1 群体为作图群体 ,从 1 0 4 0个随机引物中筛选出 78个引物 ,对 78个F1 群体及双亲样本进行了RAPD扩增 ,共获得 1 2 9个RAPD标记 ,首次构建了杉木分子遗传连锁框架图 ,其中J0亲本包含 8个连锁群 ,标记覆盖的基因组总长度约为 595.2cM ,F1 1 亲本包含 4个连锁群 ,标记覆盖的基因组总长度约为 31 5.3cM。 1 2 9个RAPD标记中偏分离标记占 1 4.7%。本图谱为构建饱和的杉木分子遗传图谱提供了框架结构 ,为进一步开展杉木分子遗传方面的研究奠定了基础。

Chinese fir ( Cunninghamia lanceolata ( Lamb.) Hook) is a fast growing evergreen coniferous tree species with wide distribution areas which cover all the subtropical in southern China. Research Group at Nanjing Forestry University made the initial work of genetic improvement of Chinese fir in the early 1960. With the 40 years effort for genetic improvement in Chinese fir, we have made great achievements in the establishment of seed orchard, provenance test, progeny test, hybridization, clonal forestry, joint genetic improvement for growth and wood properties etc. In order to accelerate the process of genetic improvement in Chinese fir and combine the new technique of molecular genetic marker with the methods of traditional forest genetics and breeding, we used F 1 progeny from cross J 0×F 11 as mapping population. 78 primers were screened from 1?040 random 10 base primers. The RAPD amplifications of both 78 F 1 samples and two parents were made. Of the 129 RAPD markers we obtained, most of them were fit for 1∶1 segregation ratio. The distortion segregation ratio was 14.7%. The first molecular genetic linkage frame map of Chinese fir was constructed. The J 0 parent identified 8 linkage groups covering 595.2?cM, the F 11 parent with 4 linkage groups covering 315.3?cM. This layed a solid basis for further molecular genetics research of Chinese fir.