水牛慢性实验感染肝片吸虫对安替比林代谢动力学的影响

The effects of chronic infection of Fasciola hepatica on the metabolism and pharmacokinetics of antipyrine in water buffaloes

5头健康雄性去势水牛 (2～ 3岁、体重 30 0～ 5 0 0kg) ,经粪便检查和Dot ELISA检测确认无肝片吸虫感染。每头每天口服 6 0个肝片吸虫囊蚴 ,连续 2 0d ,共感染 12 0 0个囊蚴 ,用以研究水牛慢性感染 (少量多次感染 )肝片吸虫对安替比林代谢动力学的影响。每周定时由颈静脉采血 ,测定血清酶水平变化。于感染前、感染后急性期及慢性期进行安替比林动力学试验。由颈静脉瘘管收集血样 ,用尿液收集器收集尿样 ,用HPLC法分析血浆安替比林动力学参数及尿安替比林清除率。结果表明 :水牛慢性实验性感染肝片吸虫呈亚临床状态 ,AP的血浆及尿代谢物清除率在急性期分别下降了48%和 6 1 91% ,慢性期逐步恢复。

Five healthy male testictomy water buffaloes of 2?3 years old with 300?500 kg body weight and no fasciolosis confirmed by fecal examination and Dot?ELISA were fed.Each one was dosed orally with 60 Fasciola hepatica metacercariae daily during 20 days to evaluate the effects of chronic infection on antipyrine metabolism.The concentrations of antipyrine in plasma and its metabolites in urine were determined by HPLC.The results showed that antipyrine plasma clearance and its metabolites clearance in urine decreased 48% and 61 91% in the acute period after Fasciola hepatica infection chronically in water buffaloes,but no obvious changes in chronic period.