嗜水气单胞菌菌种的限定代次及保存条件研究

The usage passage and storage condition of Aeromonas hydrophila stock strain

将嗜水气单胞菌(Aeromonashydrophila ,Ah)J 1菌株第 5代菌种通过鲫鱼体内复壮后 ,制备基础种子。将基础种子在普通营养琼脂培养基上连续传至 10代 ,分别测第 1代和第 10代的培养特性、免疫原性以及其毒力 ;同时 ,对Ah菌种的保存条件进行了优化。结果嗜水气单胞菌菌种在 1～ 10代之内 ,其培养特性、免疫原性以及毒力完全相同 ,表明嗜水气单胞菌基础种子的使用限定代次至少可达 10代。Ah菌种以 15 %甘油肉汤、 - 2 0℃保存为佳 ,保存期可达两年。

Aeromonas hydrophila (Ah)J 1 stock strain was serially passaged from passage 1 to passage 10 in aga culture.The biochemical characterization,immunogenicity and virulence didn′t changed.With the formalinized whole cell bacteria(WCB)of J 1(1),J 1(10)immunizing Carrassius auratus ibelio,the relative per cent survival showed no significant difference.With the optimization of storage condition to J 1 strain,the storage of Ah J 1 bacteria in 15% glycerin broth could last two years.