磁导靶向给药在荷瘤小鼠体内分布特性的实验研究

Experimental investigation of distributional characteristics in in-vivo tumor-bearing mice with magnetically targeted-drug

目的考察经磁导靶向给药载阿霉素金磁复合微粒在荷瘤小鼠体内分布特性。方法建立小鼠肝细胞癌动物模型,将30只小鼠分为三组,每组10只,空白对照组经尾缘静脉注射生理盐水,单纯磁阿霉素组和磁阿霉素加磁场组分别注射载阿霉素金磁复合微粒溶液,其中,磁阿霉素加磁场组外加5000 Gs磁场固定1 h,然后处死全部小鼠,心脏取血并收集心、肝、肾、肿瘤组织,应用高相液相色谱法测定各组织中阿霉素浓度,评价其在小鼠体内靶向分布特性。结果肿瘤靶区应用外磁场固定后,肿瘤组织中药物浓度远高于非磁区其他组织中药物浓度(P<0.05),其他组织的药物浓度(除心脏外)与空白对照组相当(P>0.05)。而在没有外磁场的环境下,药物在全身各组织中呈弥散性分布,且肝脏和肿瘤组织中药物浓度较高。结论磁导靶向给药具有良好的靶向定位作用,有望成为一种新的化疗药物剂型用于肿瘤的靶向治疗。

Objective To study the distribution of Doxorubicin loaded with GoldMag Nanaparticles in mice with hepatocellular carcinoma. Methods 30 tumor-bearing mice models were established and divided into three groups, each with 10 mice. The control group was given saline by trailing edge; the group which was administered doxorubicin-loaded GoldMag Nanaparticles was recorded as the Mag-Doxorubicin group; and the group which was given an 5000 Gs external magnetic field for 1 hour on the basis of the Mag-Doxorubicin group was recorded as the Mag-Doxorubicin＋ magnetic field. Then, all mice were killed. Hearts, livers, kidneys, solid tumors, and blood in heart were collected. HPLC was adopted to determine the concentration of doxorubicin in different tissues and evaluated the in-vivo distribution. Results The drug concentration in tumor given an external magnetic field was much higher than other tissues not given an external magnetic field （P 〈 0.05）, the drug concentration in tissues not given an external magnetic field had no significantly difference from the control group （P 〉 0.05）. When an external magnetic field was not given, the drug diffusely distributed into all tissues with higher in liver and solid tumor. Conclusion Magnetically targeted-drug has satisfied targeting effect and could be a new treatment for tumor.