胶束电动毛细管电泳法分析吡咯并喹啉醌二甲酯与氨基酸反应行为学

Determination of PQQZ and study on the reaction behavior between PQQZ and amino acids by micellar electrokinetic capillary electrophoresis

采用胶束电动毛细管电泳法分析了吡咯并喹啉醌二甲酯的纯度。以表面活性剂十二烷基硫酸钠（SDS）加入到缓冲液体系形成胶束，并加入有机改性试剂，使吡咯并喹啉醌二甲酯（PQQZ）与其他难分离的电中性杂质达到高效快速的分离。运行缓冲溶液为：50mmol／L磷酸盐-50mmol／L硼酸缓冲液-10mmol／LSDS混合液（pH7．0），含体积分数10％乙腈。在0．0125—0．2mg／mL范围内呈线性，回归方程为Y=1×10^6ρ＋108761（R^2=0．9965）。检出限为0．1μmol／L（S／N≥3）。通过PQQZ与D—Serine的化学反应分析了PQQZ是否同样对氨基酸具有调节作用，为药物设计提供实验依据。

The content of PQQZ was determined by micellar electrokinetic capillary electrophoresis PQQZ was separated from other neutral impurities using SDS and organic reagents as improved reagents. Running buffer is： 50 mmol/L phosphate - 50 mmol/L boric acid buffer - 10 mmol/L SDS, pH 7. 0, 10% acetonitrile. Excellent linear correlation was showed in the range of 0. 0125 - 0. 2 mg/mL. The regression equation was Y=1×10^6ρ＋108761（R^2=0.9965）. The detection limit for PQQZ was 0. 1 μmol/L （ S/N ≥ 3）. This method has advantages of low detection limit, little sample （nL） and low reagent consumption. Furthermore, to investigate the effects of PQQZ on in regulating neurotransmitters, our laboratory studied the reaction behavior between PQQZ and D-Serine was studied. This work can provide experimental basis for drug design.