离子选择性电极技术快速检测透明质酸钠

Potentiometric measurement of Sodium Hyaluronate by using a polymeric membrane ion-selective electrode

以聚阴离子透明质酸钠为研究对象，以二壬基萘磺酸为阳离子交换剂，发展了基于鱼精蛋白可控释放的聚阳离子选择性电极，实现了透明质酸钠的快速、高灵敏检测。详细研究了亲脂性有机盐四（4一氯苯基）硼酸四（十二烷基）铵对电极响应性能的影响。在最佳条件下，电极对透明质酸钠的检出限为0．05μg／mL，线性范围为0．1～5μg/mL。

A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of sodium hyaluronate （SH）. The incorporation of tetradodecylammonium tetrakis （4-ehlorophenyl）borate as a lipophilic salt in the membrane dramatically improves selectivity of the sensor selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane and a stable potential baseline is obtained in the presence of an electrolyte background. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear range of 0. 1 - 5 relationship between the initial slope of the potential change and the SH concentration in the μg/mL with an improved detection limit of 0. 05 μg/mL.