高效液相色谱法测定配制酒中的6种芳香物质

Determination of six aromatic components in blending liquor by high performance liquid chromatography

建立了同时测定配制酒中6种常见芳香物质（苯甲醇、β-苯乙醇、苯甲醛、苯乙酮、丁香醛、肉桂醛）的高效液相色谱法。样品稀释后，用HLB固相柱萃取净化，甲醇洗脱。采用ZobaxSB-C18色谱柱（4．6mm×150mm，5．0μm），以甲醇和水作为流动相，梯度洗脱。在1．0—20．0mg／L质量浓度范围内线性相关良好，线性相关系数彤大于0．9998。各组分回收率在87．0％～105．6％之间，相对标准偏差为0．3％～2．1％。

A High performance liquid chromatographic method was established for the simultaneous determination of six aromatic components including benzylalcohol, β-phenylethanol, benzaldehyde, acetophenone, syringaldehyde and einnamaldehyde in blending liquor. After diluted, samples were purified on a HLB SPE column and eluted by methanol six aromatic components were separated on a Zobax SB-C18 Column （4.6 × 150 mm,5.0 μm）by a gradient elution with methanol and water. The recoveries were from 87.0% to 105.6% with the RSDs between 0. 3% and 2. 1%. Good linearity was obtained for the six components in the concentration range of 1.0 mg/L - 20. 0 mg/L with the linear correlation coefficients more than 0. 9998.