输注体外诱导的同种反应性淋巴细胞诱导大鼠肝脏移植免疫耐受的研究

Study on the Effect of Infusion of Alloreactive Lymphocytes Induced in vitro to Induce Liver Transplantation Tolerance in Rats

目的观察输注体外诱导的同种反应性淋巴细胞,是否可以延长大鼠移植肝脏的存活时间,从而探索一条诱导肝移植免疫耐受的新途径。方法以供者Lewis大鼠淋巴细胞作为刺激物,体外刺激受者BN大鼠淋巴细胞增殖,应用CFSE(羧基荧光素二醋酸盐琥珀酰亚胺酯)标记法检测增殖情况;将增殖后的BN大鼠淋巴细胞经照射灭活后输注给同品系的BN大鼠,并行Lewis到BN的肝移植,观察肝移植后BN大鼠生存期。结果同种反应性淋巴细胞体外能够活跃增殖;采用同种反应性淋巴细胞输注可以显著延长肝移植大鼠的存活时间(P<0.05)。结论体外诱导的同种反应性淋巴细胞输注,有可能成为诱导抗原特异性免疫耐受的一种新途径。

Objective To investigate whether infusion of alloreactive lymphocytes induced in vitro can prolong the survival of liver allograft in rats,so as to find out a new method to induce liver transplantation tolerance.Method Lymphocytes from Lewis rat were used as stimulating cells,lymphocytes from BN rat were stimulated by these stimulating cells and expanded in vitro; the proliferation of BN lymphocytes were determined by CFSE method.The proliferated BN lymphocytes were used as idiotype vaccine to vaccinate BN rats,then liver allograft from Lewis rats were transplanted to BN rats.Result Alloreactive lymphocytes proliferated vigorously in vitro; infusion of alloreactive lymphocytes induced in vitro can prolong the survival of liver allograft significantly in rats.Conclusion infusion of alloreactive lymphocytes induced in vitro may be a new method to induce donor specific transplantation tolerance.