HPLC法测定穿白痢康丸中穿心莲内酯和脱水穿心莲内酯的含量

Determination of Andrographolide and Dehydroandrographolide in Chuanbai Likang Pills by HPLC

采用液相色谱法测定穿白痢康丸中穿心莲内酯、脱水穿心莲内酯的含量。以甲醇-水(52∶48)为流动相;lnertsilODS-SP(250 mm×4.6 mm,5μm)为固定相;检测波长240 nm。穿心莲内酯在0.06-1.2μg,脱水穿心莲内酯在0.04-0.80μg范围内具有良好的线性关系;穿心莲内酯的平均加样回收率为98.6%,RSD为0.47%,脱水穿心莲内酯的平均加样回收率为99.3%,RSD为0.77%。该法简便、快速、准确可靠,可用于穿白痢康丸中穿心莲内酯和脱水穿心莲内酯的含量测定。

To establish a method for the determination of andrographolide and dehydroandrographolide in chuan bai li kang pills by HPLC. The mobile phase was methanol -water（52：48 ）. Chromatography was performed on a lnertsil~ ODS - SP （250 mm ~ 4.6 mm, 5 txm）. The detective wavelength was 240 nm. The linear range of the determination of andrographolide was 0.06 - 1.2 txg , while that for dehydroandrographolide was 0.04 - 0.80 tLg. The average recovery of andrographolide was 98. 6%, and RSD was 0. 47%. The average recovery of dehydroandrographolide was 99. 3%, and RSD was 0. 77%. The method is simple, rapid and accurate and precise,which can be used for the determination of andrographolide and dehydroandrographolide in chuan bai li kang pills.